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KMID : 0903520060490030196
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2006 Volume.49 No. 3 p.196 ~ p.201
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Reactivity of the Antibodies against Purified Carp Vitellogenin and a Synthetic Vitellogenin Peptide
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Moon Dae-Kyung
Kim Nam-Soo
Kim Woo-Yeon
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Abstract
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Vitellogenin, which is found in the serum of female and male fishes exposed to environmental endocrine disrupter or estrogen hormone, is used as a biomarker for environmental contamination with an endocrine disrupter. In order to produce antibody against vitellogenin, a synthetic peptide for partial vitellogenin was injected into rabbits. In addition, by using ion exchange chromatography on DE-52, vitellogenin was purified from the serum of carp induced with 17β-estradiol. Polyclonal antibody against purified vitellogenin reacted well with vitellogenin in the serum of carp induced with 17β-estradiol and the serum of female carp, whereas polyclonal antibody against the vitellogenin peptide did not react with proteins in those samples. This may indicate that vitellogenin proteins, covalently modified largely, could not be detected by Western blotting with the polyclonal antibody against the synthetic vitellogenin peptide.
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KEYWORD
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carp, 17β-estradiol, vitellogenin purification, peptide, antibody
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